Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues.
Identifieur interne : 001818 ( Main/Exploration ); précédent : 001817; suivant : 001819Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues.
Auteurs : Miyuki Takeuchi [Japon] ; Keiji Takabe [Japon] ; Yoshinobu Mineyuki [Japon]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2016.
Descripteurs français
- KwdFr :
- Anticorps (composition chimique), Cellules végétales (métabolisme), Cellules végétales (ultrastructure), Coloration et marquage (méthodes), Congélation-dissolution (méthodes), Cryoconservation (méthodes), Expression des gènes (MeSH), Fixateurs (composition chimique), Fixation tissulaire (méthodes), Glutaraldéhyde (composition chimique), Graines (métabolisme), Graines (ultrastructure), Immunohistochimie (méthodes), Inclusion de tissu (méthodes), Membrane cellulaire (métabolisme), Membrane cellulaire (ultrastructure), Microscopie immunoélectronique (méthodes), Microtomie (MeSH), Oignons (métabolisme), Oignons (ultrastructure), Populus (métabolisme), Populus (ultrastructure), Protéines végétales (génétique), Protéines végétales (métabolisme), Protéines à homéodomaine (génétique), Protéines à homéodomaine (métabolisme), Résines époxy (composition chimique), Tiges de plante (métabolisme), Tiges de plante (ultrastructure), Tubuline (génétique), Tubuline (métabolisme).
- MESH :
- composition chimique : Anticorps, Fixateurs, Glutaraldéhyde, Résines époxy.
- génétique : Protéines végétales, Protéines à homéodomaine, Tubuline.
- métabolisme : Cellules végétales, Graines, Membrane cellulaire, Oignons, Populus, Protéines végétales, Protéines à homéodomaine, Tiges de plante, Tubuline.
- méthodes : Coloration et marquage, Congélation-dissolution, Cryoconservation, Fixation tissulaire, Immunohistochimie, Inclusion de tissu, Microscopie immunoélectronique.
- ultrastructure : Cellules végétales, Expression des gènes, Graines, Membrane cellulaire, Microtomie, Oignons, Populus, Tiges de plante.
English descriptors
- KwdEn :
- Antibodies (chemistry), Cell Membrane (metabolism), Cell Membrane (ultrastructure), Cryopreservation (methods), Epoxy Resins (chemistry), Fixatives (chemistry), Freeze Substitution (methods), Gene Expression (MeSH), Glutaral (chemistry), Homeodomain Proteins (genetics), Homeodomain Proteins (metabolism), Immunohistochemistry (methods), Microscopy, Immunoelectron (methods), Microtomy (MeSH), Onions (metabolism), Onions (ultrastructure), Plant Cells (metabolism), Plant Cells (ultrastructure), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Stems (metabolism), Plant Stems (ultrastructure), Populus (metabolism), Populus (ultrastructure), Seeds (metabolism), Seeds (ultrastructure), Staining and Labeling (methods), Tissue Embedding (methods), Tissue Fixation (methods), Tubulin (genetics), Tubulin (metabolism).
- MESH :
- chemical , chemistry : Antibodies, Epoxy Resins, Fixatives, Glutaral.
- chemical , genetics : Homeodomain Proteins, Plant Proteins, Tubulin.
- metabolism : Cell Membrane, Homeodomain Proteins, Onions, Plant Cells, Plant Proteins, Plant Stems, Populus, Seeds, Tubulin.
- methods : Cryopreservation, Freeze Substitution, Immunohistochemistry, Microscopy, Immunoelectron, Staining and Labeling, Tissue Embedding, Tissue Fixation.
- ultrastructure : Cell Membrane, Onions, Plant Cells, Plant Stems, Populus, Seeds.
- Gene Expression, Microtomy.
Abstract
Cryofixation and freeze-substitution techniques provide excellent preservation of plant ultrastructure. The advantage of cryofixation is not only in structural preservation, as seen in the smooth plasma membrane, but also in the speed in arresting cell activity. Immunoelectron microscopy reveals the subcellular localization of molecules within cells. Immunolabeling in combination with cryofixation and freeze-substitution techniques provides more detailed information on the immunoelectron-microscopic localization of molecules in the plant cell than can be obtained from chemically fixed tissues. Here, we introduce methods for immunoelectron microscopy of cryofixed and freeze-substituted plant tissues.
DOI: 10.1007/978-1-4939-6352-2_14
PubMed: 27515084
Affiliations:
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last="Takeuchi">Miyuki Takeuchi</name><affiliation wicri:level="4"><nlm:affiliation>Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyou-ku, Tokyo, 113-8657, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyou-ku, Tokyo, 113-8657</wicri:regionArea><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Takabe, Keiji" sort="Takabe, Keiji" uniqKey="Takabe K" first="Keiji" last="Takabe">Keiji Takabe</name><affiliation wicri:level="4"><nlm:affiliation>Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Forest and Biomaterials 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Substitution</term><term>Immunohistochemistry</term><term>Microscopy, Immunoelectron</term><term>Staining and Labeling</term><term>Tissue Embedding</term><term>Tissue Fixation</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules végétales</term><term>Graines</term><term>Membrane cellulaire</term><term>Oignons</term><term>Populus</term><term>Protéines végétales</term><term>Protéines à homéodomaine</term><term>Tiges de plante</term><term>Tubuline</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Coloration et marquage</term><term>Congélation-dissolution</term><term>Cryoconservation</term><term>Fixation tissulaire</term><term>Immunohistochimie</term><term>Inclusion de tissu</term><term>Microscopie immunoélectronique</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Cell Membrane</term><term>Onions</term><term>Plant Cells</term><term>Plant Stems</term><term>Populus</term><term>Seeds</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression</term><term>Microtomy</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Cellules végétales</term><term>Expression des gènes</term><term>Graines</term><term>Membrane cellulaire</term><term>Microtomie</term><term>Oignons</term><term>Populus</term><term>Tiges de plante</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cryofixation and freeze-substitution techniques provide excellent preservation of plant ultrastructure. The advantage of cryofixation is not only in structural preservation, as seen in the smooth plasma membrane, but also in the speed in arresting cell activity. Immunoelectron microscopy reveals the subcellular localization of molecules within cells. Immunolabeling in combination with cryofixation and freeze-substitution techniques provides more detailed information on the immunoelectron-microscopic localization of molecules in the plant cell than can be obtained from chemically fixed tissues. Here, we introduce methods for immunoelectron microscopy of cryofixed and freeze-substituted plant tissues.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27515084</PMID><DateCompleted><Year>2018</Year><Month>01</Month><Day>02</Day></DateCompleted><DateRevised><Year>2018</Year><Month>04</Month><Day>03</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1474</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues.</ArticleTitle><Pagination><MedlinePgn>233-42</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-6352-2_14</ELocationID><Abstract><AbstractText>Cryofixation and freeze-substitution techniques provide excellent preservation of plant ultrastructure. The advantage of cryofixation is not only in structural preservation, as seen in the smooth plasma membrane, but also in the speed in arresting cell activity. Immunoelectron microscopy reveals the subcellular localization of molecules within cells. Immunolabeling in combination with cryofixation and freeze-substitution techniques provides more detailed information on the immunoelectron-microscopic localization of molecules in the plant cell than can be obtained from chemically fixed tissues. Here, we introduce methods for immunoelectron microscopy of cryofixed and freeze-substituted plant tissues.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Takeuchi</LastName><ForeName>Miyuki</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyou-ku, Tokyo, 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Takabe</LastName><ForeName>Keiji</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mineyuki</LastName><ForeName>Yoshinobu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Picobiology, Graduate School of Life Science, University of Hyogo, 2167 Shosha, Himeji, Hyogo, 671-2280, Japan. mineyuki@sci.u-hyogo.ac.jp.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000906">Antibodies</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004853">Epoxy Resins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005404">Fixatives</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018398">Homeodomain Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014404">Tubulin</NameOfSubstance></Chemical><Chemical><RegistryNumber>T3C89M417N</RegistryNumber><NameOfSubstance UI="D005976">Glutaral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000906" MajorTopicYN="N">Antibodies</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015925" MajorTopicYN="N">Cryopreservation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004853" MajorTopicYN="N">Epoxy Resins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005404" MajorTopicYN="N">Fixatives</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017110" MajorTopicYN="N">Freeze Substitution</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005976" MajorTopicYN="N">Glutaral</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018398" MajorTopicYN="N">Homeodomain Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007150" MajorTopicYN="N">Immunohistochemistry</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016253" MajorTopicYN="N">Microscopy, Immunoelectron</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008867" MajorTopicYN="N">Microtomy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019697" MajorTopicYN="N">Onions</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013194" MajorTopicYN="N">Staining and Labeling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016610" MajorTopicYN="N">Tissue Embedding</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016707" MajorTopicYN="N">Tissue Fixation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014404" MajorTopicYN="N">Tubulin</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Cryofixation</Keyword><Keyword MajorTopicYN="Y">Freeze substitution</Keyword><Keyword MajorTopicYN="Y">High-pressure freezing</Keyword><Keyword MajorTopicYN="Y">Immunoelectron microscopy</Keyword><Keyword MajorTopicYN="Y">Plant tissue</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>8</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>8</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>1</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27515084</ArticleId><ArticleId IdType="doi">10.1007/978-1-4939-6352-2_14</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li><li>Région du Kansai</li></region><settlement><li>Kyoto</li><li>Tokyo</li></settlement><orgName><li>Université de Kyoto</li><li>Université de Tokyo</li></orgName></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Takeuchi, Miyuki" sort="Takeuchi, Miyuki" uniqKey="Takeuchi M" first="Miyuki" last="Takeuchi">Miyuki Takeuchi</name></region><name sortKey="Mineyuki, Yoshinobu" sort="Mineyuki, Yoshinobu" uniqKey="Mineyuki Y" first="Yoshinobu" last="Mineyuki">Yoshinobu Mineyuki</name><name sortKey="Takabe, Keiji" sort="Takabe, Keiji" uniqKey="Takabe K" first="Keiji" last="Takabe">Keiji Takabe</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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